EpiCypher是一家為表觀遺傳學和染色質生物學研究提供高質量試劑和工具的專業制造商。EpiCypher能提供全長重組人SMARCA2重塑酶。SMARCA2是ATP依賴性染色質重塑復合物SWI/SNF的核心ATP酶亞基，可動員核小體，在基因轉錄調控、發育、基因組復制和修復中發揮關鍵作用[1,2]。SWI/SNF亞基的異常活性與超過20%的人類癌癥和多種發育障礙相關，這使得SMARCA2成為一個新興的候選治療靶點[3,4]。SMARCA2可用于結合EpiDyne®-FRET Remodeling Assay Substrate (EpiCypher 16-4201)在HTS兼容藥物發現研究。

[1] Clapier & Carins Annu. Rev. Biochem. (2009). PMID: 19355820

[2] Sundaramoorthy & Owen-Hughes F1000Research (2020). PMID: 32864100

[3] Kadoch & Crabtree Sci. Adv. (2015). PMID: 26601204

[4] Cenik & Shilatifard Nat. Rev (2021). PMID: 32958894

產品詳情

宿主來源: Human

表達系統: SF9 cells

保存溫度: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid freeze/thaws.

運輸溫度: Dry ice.

產品形式: SMARCA2 at 0.175 mg/mL in 20.7 µL of 25 mM HEPES pH 7.6, 0.1 mM EDTA, 10% glycerol, 350 mM NaCl, 1 mM DTT, 0.4 mM PMSF, 1 mM benzamidine, 0.02% NP-40, 0.4 mg/mL insulin

驗證數據

Figure 1: ATP-dependent chromatin remodeling assay EpiDyne®-FRET Chromatin Remodeling Substrate (EpiCypher 16-4201; 20 nM) incubated with SMARCA2 Remodeling Enzyme (concentrations indicated) in 1x SMARCA Remodeling Assay Buffer. Curves denote FRET efficiency/chromatin remodeling.

Figure 2: Protein gel data SMARCA2 Remodeling Enzyme (1 µg) was run on an SDS-PAGE gel and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated.

訂購詳情

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