Enzo Life Sciences的ROS-ID® Superoxide detection kit可利用熒光顯微鏡和/或流式細胞儀直接實時監測活細胞中超氧化物的產生。該試劑盒的一個主要成分，超氧化物檢測試劑（橙色），是一種細胞滲透性探針，可與超氧化物發生特異性反應，產生橙色熒光產物。熒光探針對活性過氧化物、羥基、過氧亞硝酸鹽、氯或溴相對不敏感，因此該試劑盒不能檢測這些分析物。染色后，可使用配備有標準橙色（如550/620 nm）濾光片的寬場顯微鏡進行觀察，或使用配備有藍色（488 nm）激光的流式細胞儀進行分析。

超氧化物檢測試劑盒包含足夠的試劑，可使用活細胞（貼壁或懸浮）進行至少200次顯微鏡分析或50次流式細胞術分析。

產品特點

可通過熒光顯微鏡或流式細胞術直接監測活細胞中超氧化物的整體水平

高靈敏度、特異性和準確性，可用于活細胞研究

與組織培養基的主要成分（酚紅、FBS和BSA）兼容

試劑盒內包括整套所需試劑，包括ROS誘導劑和清除劑

產品信息

部分產品引用文獻

1. Rational design of mitochondria targeted thiabendazole-based Ir (III) biscyclometalated complexes for a multimodal photodynamic therapy of cancer: I. Echevarria, et al.; J. Inorg. Biochem. 231, 111790 (2022)

2. A spatiotemporal characterisation of redox molecules in planarians, with a focus on the role of glutathione during regeneration: K. Bijnens, et al.; Biomolecules 11, 714 (2021)

3. An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination: Y. Wang, et al.; Nat. Commun. 12, 4964 (2021)

4. Assay for advanced glycation end products generating intracellular oxidative stress through binding to its receptor: T. Kobori, et al.; Anal. Biochem. 611, 114018 (2020)

5. Epithelial cadherin regulates transition between the na?ve and primed pluripotent states in mouse embryonic stem cells: A.M. Sharaireh, et al.; Stem Cells 38, 1292 (2020)

6. Loss of Spry1 reduces growth of BRAFV600-mutant cutaneous melanoma and improves response to targeted therapy: B. Montico, et al.; Cell Death Dis. 11, 392 (2020)

7. PCSK9 expression in the ischemic heart and its relationship to infarct size, cardiac function and development of autophagy: Z. Ding, et al.; Cardiovasc. Res. 114, 1738 (2018)

8. SPARC paucity alleviates superoxide-mediated oxidative stress, apoptosis, and autophagy in diabetogenic hepatocytes: K.R. Aseer, et al.; Free Radic. Biol. Med. 108, 874 (2017)

9. Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression: L. Pasterk, et al.; Sci. Rep. 6, 22104 (2016)

10. TRPC6 channel activation promotes neonatal glomerular mesangial cell apoptosis via calcineurin/NFAT and FasL/Fas signaling pathways: H. Soni, et al.; Sci. Rep. 6, 29041 (2016)

11. Hemodynamic Shear Stress via ROS Modulates PCSK9 Expression in Human Vascular Endothelial and Smooth Muscle Cells and Along the Mouse Aorta: Z. Ding, et al.; Antioxid. Redox Signal. 22, 760 (2015)

12. Influenza A virus PB1-F2 is involved in regulation of cellular redox state in alveolar epithelial cells: N. Shin, et al.; Biochem. Biophys. Res. Commun. 459, 699 (2015)

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